Japanese Management Strategies

During the detailed researching work of the Kaizen based management practices of the most advanced Japanese companies, that is the best representatives of the Japanese industry, at certain phases occures the need to have a look of a wider perspective embracing some aspects of the strategies and the external connections of these firms, especially the lean enterprises.The goal of this paper is giving a framework for the detailed researches investigating the Kaizen based activities within the companies with the help of general pictures on the ‘Japanese way’ and on the behaviour of the Japanese companies in the glorious fast growth period and then in the times of the serious crises and stagnation as an adaptation to the globalization process.Japanese company culture, Kaizen management philosophy, lean enterprise, corporate strategy,confrontation strategy, avoiding strategy, organizational learning

Shtylo : stilometriai elemzések webes támogatása

A stilometria a számítógépes nyelvészet dinamikusan fejlődő területe. Széles körű felhasználását azonban gátolja az a tény, hogy alkalmazóinak többsége nincs a szükséges informatikai tudás birtokában. Cikkünkben egy olyan rendszert mutatunk be, amely az R nyelven írt stylo programcsomaghoz nyújt egy teljes értékű webes felhasználói felületet, valamint segítséget nyújt a stilometriai kísérletekhez szükséges korpuszok összeállításában és tárolásában is. Az elkészített szoftver működését egyrészt történeti szövegek elemzésén, másrészt plágiumkeresési feladat végrehajtásán mutatjuk be

Rapid in silico selection of an MCHR1 antagonists' focused library from multi-million compounds' repositories : Biological evaluation

Target-focused libraries can be rapidly selected by 2D virtual screening methods from multimillion compounds' databases if structures of active compounds are available. In the present study, a multi-step virtual and in vitro screening cascade is used to select melanin-concentrating hormone receptor-1 antagonists. The 2D similarity search combined with physico-chemical parameter filtering is suitable for selecting candidates from multimillion compounds' repository. The seeds of the first round of virtual screening were collected from literature commercial databases, whereas the seeds of the second round were the hits the first round of biological testing In vitro screening underlined the efficiency of our approach, as in the second screening round the hit rate (8.6 %) significantly improved compared to the first round (1.9 %), applying a strict requirement for hit selection, and also this cascade-like screening method was appropriate for selecting several compounds reaching efficacies even below 10 nM. © 2013 Springer Science+Business Media New York

Robust Recombinant Expression of Human Placental Ribonuclease Inhibitor in Insect Cells

Ribonuclease inhibitors (RIs) are an indispensable biotechnological tool for the detection and manipulation of RNA. Nowadays, due to the outbreak of COVID-19, highly sensitive detection of RNA has become more important than ever. Although the recombinant expression of RNase inhibitors is possible in E. coli, the robust expression is complicated by maintaining the redox potential and solubility by various expression tags. In the present paper we describe the expression of RI in baculovirus-infected High Five cells in large scale utilizing a modified transfer vector combining the beneficial properties of Profinity Exact Tag and pONE system. The recombinant RI is expressed at a high level in a fusion form, which is readily cleaved during on-column chromatography. A subsequent anion exchange chromatography was used as a polishing step to yield 12 mg native RI per liter of culture. RI expressed in insect cells shows higher thermal stability than the commercially available RI products (mainly produced in E. coli) based on temperature-dependent RNase inhibition studies. The endotoxin-free RI variant may also be applied in future therapeutics as a safe additive to increase mRNA stability in mRNA-based vaccines

Monoclonal antibody proteomics: Use of antibody mimotope displaying phages and the relevant synthetic peptides for mAb scouting

Monoclonal antibody proteomics uses nascent libraries or cloned (Plasmascan™, QuantiPlasma™) libraries of mAbs that react with individual epitopes of proteins in the human plasma. At the initial phase of library creation, cognate protein antigen and the epitope interacting with the antibodies are not known. Scouting for monoclonal antibodies (mAbs) with the best binding characteristics is of high importance for mAb based biomarker assay development. However, in the absence of the identity of the cognate antigen the task represents a challenge. We combined phage display, and surface plasmon resonance (Biacore) experiments to test whether specific phages and the respective mimotope peptides obtained from large scale studies are applicable to determine key features of antibodies for scouting. We show here that mAb captured phage-mimotope heterogeneity that is the diversity of the selected peptide sequences, is inversely correlated with an important binding descriptor; the off-rate of the antibodies and that represents clues for driving the selection of useful mAbs for biomarker assay development. Carefully chosen synthetic mimotope peptides are suitable for specificity testing in competitive assays using the target proteome, in our case the human plasma. © 2014 Elsevier B.V. All rights reserved

Monoclonal antibody proteomics: use of antibody mimotope displaying phages and the relevant synthetic peptides for mAb scouting.

Monoclonal antibody proteomics uses nascent libraries or cloned (Plasmascan™, QuantiPlasma™) libraries of mAbs that react with individual epitopes of proteins in the human plasma. At the initial phase of library creation, cognate protein antigen and the epitope interacting with the antibodies are not known. Scouting for monoclonal antibodies (mAbs) with the best binding characteristics is of high importance for mAb based biomarker assay development. However, in the absence of the identity of the cognate antigen the task represents a challenge. We combined phage display, and surface plasmon resonance (Biacore) experiments to test whether specific phages and the respective mimotope peptides obtained from large scale studies are applicable to determine key features of antibodies for scouting. We show here that mAb captured phage-mimotope heterogeneity that is the diversity of the selected peptide sequences, is inversely correlated with an important binding descriptor; the off-rate of the antibodies and that represents clues for driving the selection of useful mAbs for biomarker assay development. Carefully chosen synthetic mimotope peptides are suitable for specificity testing in competitive assays using the target proteome, in our case the human plasma

Monoclonal antibody proteomics: use of antibody mimotope displaying phages and the relevant synthetic peptides for mAb scouting

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